Detection of Activated Mouse Neurons with Temporal Resolution via Dual c-Fos Staining

Detection of Activated Mouse Neurons with Temporal Resolution via Dual c-Fos Staining

Blog Article

Summary: This educational toys protocol combines fluorescent in situ hybridization and immunostaining to simultaneously detect, in histological sections from the same animal, subpopulations of neurons activated after two episodes of sensory stimulation.It allows the identification of groups of cells singly activated by either stimulus or co-activated by both stimuli.Our method results in nuclear staining for c-Fos mRNA and c-Fos protein, allowing better spatial and temporal resolution than previously published protocols, Building Set although it requires quick brain fixation.

For complete details on the use and execution of this protocol, please refer to Carvalho et al.(2015, 2020).